Journal: Vaccines

Article Title: Jet Injection of Naked mRNA Encoding the RBD of the SARS-CoV-2 Spike Protein Induces a High Level of a Specific Immune Response in Mice

doi: 10.3390/vaccines13010065

Figure Lengend Snippet: Parameters of different jet injection protocols.

Article Snippet: Fluorescence activity was assessed qualitatively one-hour post-injection using an in vivo fluorescence and bioluminescence visualization and analysis system (VISQUE InVivo Series Smart-LF, Vieworks Co., Ltd., Anyang-si, Republic of Korea).

Techniques: Injection, Fluorescence

Journal: Vaccines

Article Title: Jet Injection of Naked mRNA Encoding the RBD of the SARS-CoV-2 Spike Protein Induces a High Level of a Specific Immune Response in Mice

doi: 10.3390/vaccines13010065

Figure Lengend Snippet: ( A ). Fluorescence microphotographs of muscle tissue obtained 3 days after immunization by jet injection of GFP plasmid. Samples were collected and then cut into 1 mm thick slices to visualize GFP expression: ( A’ ) control mice (without jet injection); ( B’ ) protocol No. 1; ( C’ ) protocol No. 2; ( D’ ) protocol No. 3. ( B ). Fluorescence intensity was plotted using computer-generated signal processing. (A) control mice (without jet injection); (B) protocol No. 1; (C) protocol No. 2; (D) protocol No. 3. Statistical significance was determined by nonparametric Kruskal–Wallis analysis with correction for multiple comparisons and Dunn’s test for statistical hypotheses (** p < 0.01, **** p < 0.0001).

Article Snippet: Fluorescence activity was assessed qualitatively one-hour post-injection using an in vivo fluorescence and bioluminescence visualization and analysis system (VISQUE InVivo Series Smart-LF, Vieworks Co., Ltd., Anyang-si, Republic of Korea).

Techniques: Fluorescence, Injection, Plasmid Preparation, Expressing, Control, Generated